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applied biosystemstm microamptm optical 384- well reaction plate with barcode  (Thermo Fisher)


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    Thermo Fisher applied biosystemstm microamptm optical 384- well reaction plate with barcode
    Applied Biosystemstm Microamptm Optical 384 Well Reaction Plate With Barcode, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/applied+biosystemstm+microamptm+optical+384-+well+reaction+plate+with+barcode/applied+biosystemstm+microamptm+optical+384++well+reaction+plate+with+barcode/pm40473842-190-47-48
    Average 90 stars, based on 1 article reviews
    applied biosystemstm microamptm optical 384- well reaction plate with barcode - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Adhesive:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Polymerase Chain Reaction:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Mass Measurement:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Western Blot:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Imaging:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Spectrophotometry:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Nucleic Acid Electrophoresis:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..

    Microscopy:

    Article Title: Dynamic multi-omics and mechanistic modeling approach uncovers novel mechanisms of kidney fibrosis progression.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Cell culture reagents DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, L-Glutamine, 110 mg/ L Sodium Pyruvate Gibco 31885 DMEM (Dulbecco’s Modified Eagle Medium) 1 g/L Dglucose, no glutamine, no phenol red Gibco 11880 FBS (fetal bovine serum) Gibco A5256701 L-Glutamine solution Sigma Life Science G7513 Ficoll® PM 770 kDa Sigma-Aldrich F2878 Ficoll® PM 400 kDa Sigma-Aldrich F4375 Recombinant Human TGFbeta 1 Protein R&D systems 240-B-010 BSA (Bovine Serum Albumin) Sigma-Aldrich A2153 L-Ascorbic Acid 2-phosphate (magnesium salt hydrate) Cayman Chemical Company 16457 Trypsin-EDTA 0.05% Gibco 25300-054 AmbionTM Nuclease-Free Water, DEPC-Treated Life Technologies Corp. AM9906 DMSO (Dimethyl sulfoxide) Sigma-Aldrich D2438 ScreenFect® Dilution Buffer ScreenFect S-2001 ScreenFect® siRNA ScreenFect S-4001 Antibodies and fluorescent dyes Collagen Type I Antibody, rabbit polyclonal Rockland 600-401- 103-0.5 Anti-GFP, rabbit polyclonal Origene TP401 phospho-SMAD2 (Ser465/ Ser467) (E8F3R), rabbit monoclonal Cell Signaling #18338 SMAD2, rabbit polyclonal Proteintech 12570-1-AP Tubulin-α AB-2, mouse monoclonal Thermo Scientific MS581 Phalloidin AlexaFluor 647 Invitrogen A22287 © The Author(s) Molecular Systems Biology 11 D ow nloaded from https://w w w .em bopress.org on June 7, 2025 from IP 2401:4900:1ce1:c974:4988:1dc5:ac88:a49a. .. Reagent/resource Reference or source Identifier or catalog number Cool Cell freezing container Corning CLS432004 Cryotubes Thermo Scientific 363401 MicroAmpTM Fast Optical 96-Well Reaction Plate Applied Biosystems by Life Technologies 4346906 MicroAmpTM Optical Adhesive Film Thermo Fisher Scientific 4311971 Applied BiosystemsTM MicroAmpTM Optical 384- Well Reaction Plate with Barcode Applied Biosystems by Life Technologies 4343814 PCR strips of 8 tubes 0–2ml Ratiolab 8610040 StepOne Real-Time PCR system Thermo Fisher Scientific QuantStudio 6 Flex Applied Biosystems by Life Technologies T100 Thermal Cycler Bio-Rad Laboratories Centrifuge 5417 R Eppendorf Centrifuge 5408 R Eppendorf Vortex-Genie 2 Scientific Industries Mini-Centrifuge ROTILABO® Carl Roth Analytical balance TE124SOCE Sartorius Tilt/roller mixer RS-TR05 Phoenix Instruments ThermoMixer C Eppendorf Scale VWR Rotary shaker neoLab Azure 280 Western blot imaging system Azure Biosystems ChemiDoc TM Touch Imaging System Bio-Rad Tissue culture incubator Binder Thermo Scientific Heraeus® BBD6220 incubator Thermo Scientific Water bath GFLR Magnetic stirring hotplate MR3001 K Heidolph Mini Trans-blot Cell Blotting system Bio-Rad Mini Trans-blot Cell system Bio-Rad Nanodrop 8000 Spectrophotometer Thermo Scientific Protein Gel Electrophoresis XCell SureLock System ThermoFisher Scientific Infinite M1000 pro plate reader Tecan Automated widefield screening microscope Molecular Devices IXM IXM Confocal high-throughput Microscope LSM 900 Zeiss No blinding was performed for the experiments. ..



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